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The development of single cell detection technology provides a means to understand the composition and function of cells in tissues. However, although the existing methods can detect the cell type, they cannot truly restore the spatial composition position in the tissue. The development of spatial transcriptomics technology provides a powerful detection method for detecting the spatial composition of cells and gene expression in tissues by combining the advantages of traditional histology technology with high-throughput RNA sequencing technology.
Single-cell transcriptome sequencing uses the microfluidic technology of GemCode ™ technology for single-cell sorting, encapsulating gel beads with barcodes and primers and individual cells in oil droplets; within each oil droplet, the gel beads dissolve After the cell is lysed to release mRNA, the barcoded cDNA for sequencing is generated by reverse transcription. After the liquid oil layer is destroyed, the cDNA is subsequently subjected to library construction and combined with the Illumina sequencing platform for sequencing and detection of the library, a large number of single-cell genes can be obtained at once Expression data to achieve the purpose of expression sequencing at the single cell level. The development of 10 × Genomics' Chromium Single Cell sequencing technology has greatly promoted single cell omics research.
Due to the research needs of tumor heterogeneity, embryonic development, stem cells, and the ecological diversity of microbial communities, the field of single cell omics has developed very rapidly. Single-cell transcriptome sequencing refers to pre-amplifying the transcriptome from the isolated single cells (or a small number of cells), and then sequencing to analyze changes in gene expression at the single-cell level.
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