Shanghai Biochip Co., Ltd.
Shanghai Biochip Co., Ltd.
- ꁕ Transcriptome Services ꁕ
- ꁕ Single cell sequencing ꁕ
- ꁕ Immunoassay analysis
- ꁕ Validation Services ꁕ
- Health letter analysis service
- ꁕ Apparent group service
- ꁕ Genome Services ꁕ
- ꁕ Molecular diagnostic products ꁕ
- ꁕ Other products ꁕ
The specific reaction between the antigen and the antibody is used to connect the test object to the enzyme, and then a color reaction is generated by the enzyme and the substrate for quantitative determination. The object to be measured may be an antibody or an antigen.
There are three necessary reagents in this assay:
①Solid phase antigen or antibody (immunoadsorbent)
②Enzyme-labeled antigen or antibody (label)
③ Substrate (chromogen) by enzyme
During the measurement, the antigen (antibody) is first bound to the solid phase carrier, but still retains its immunological activity. Then a conjugate (label) formed by binding the antibody (antigen) with the enzyme is retained, and the conjugate still retains its conjugate. Proto-immune activity and enzyme activity, when the conjugate reacts with the antigen (antibody) on the solid-phase support, and then add the corresponding substrate of the enzyme, it will catalyze the hydrolysis or redox reaction and show color.
The intensity of the color produced is directly proportional to the antigen (antibody) content to be measured. This colored product can be observed with the naked eye, light microscope, electron microscope, or it can be measured with a spectrophotometer (microplate reader). The method is simple, convenient, fast and specific.
The law can be divided into the following types by types and changes:
(I) Double antibody sandwich method
(2) Indirect method
(3) Competition law
(4) Two-site one-step method
(E) Capture IgM antibodies
(6) ELISA using avidin and biotin
The sensitivity of this assay is derived from the enzyme as a reporter group. As we all know, enzyme is an organic catalyst, a small amount of enzyme can induce a large number of catalytic reactions, resulting in observable color reaction phenomenon. Therefore this system is often called the enzyme amplification system. ELISA allows the location of antigens or antibodies to be traced at the cellular or subcellular level, or quantified at the microgram or even nanogram level.
Its specificity comes from the selectivity of antibodies or antigens. Antigen-antibody binding essentially occurs only between the antigenic determinant of the antigen and the antigen-binding site of the antibody. Because the two are complementary in chemical structure and spatial configuration, the antigen-antibody reaction is highly specific.
Copyright © Shanghai Biochip Co., Ltd.
Address: 151 Li Bing Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai
Scan to view
WeChat public account