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Digital PCR (dPCR)
An absolute quantification technique for nucleic acid molecules based on the Poisson distribution principle. Thousands of droplets are formed by a droplet generator, each of which is either free of or containing one to several nucleic acid target molecules. Each single molecule is subjected to PCR amplification, and the probe is used to detect a target of a specific sequence. Subsequently, each droplet is tested for the fluorescence signal one by one. Finally, according to the proportion of the positive droplets, and according to the principle of Poisson distribution, the copy number of the target molecule to be detected is calculated by software.
Real-time fluorescent quantitative polymerase chain reaction, using fluorescent hybridization probes, and using fluorescence signals to cumulatively monitor the amplification efficiency of PCR to accurately quantify the number of starting templates and perform quantitative analysis of specific DNA sequences using internal and external parameters.
Gene editing refers to a technical method to achieve the deletion, insertion or replacement of the DNA sequence of a specific gene in the genome.
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